Transcriptional Analysis of Maize Elite Inbred Line Jing24 and the Function of ZmMAPKKK21 in the Response to Drought Stress
Agriculture Communications（2024）
Abstract
Drought, one of the most devastating abiotic stresses to affect agricultural production worldwide, causes significant crop yield loss. In China, the yield and value of maize, a drought-sensitive staple crop, is significantly affected by drought. Jing24 (J24) is an elite, robust, and drought-resistant maize inbred line, but the underlying genetic basis for this resistance is imperfectly understood. We characterized the overall performance of three maize varieties (J24, B73, and X178) under moderate and severe drought conditions at seedling and flowering stages. RNA-Seq analysis revealed more genes to respond to drought treatment in J24 than either B73 or X178, and that some drought-responsive genes were common to each line in leaf and root tissues. Gene ontology analysis of common differentially expressed genes in J24 and X178 revealed membrane and transporter related genes to be significantly enriched in roots, whereas genes associated with photosynthesis and membrane were most-enriched in leaves. Because expression of ZmMAPKKK21 was significantly up-regulated in the root of J24 in the moderate drought treatment, we obtained transgenic Arabidopsis plants overexpressing ZmMAPKKK21 that showed a substantial reduction in ABA sensitivity but increase in drought tolerance. Maize plants in which ZmMAPKKK21 was knocked out were more sensitive to water deficiency and have a smaller root system and a lower survival rate after rewatering than wild type plants. These results suggest that ZmMAPKKK21 is a positive regulator for drought response in J24, which provides insights into the molecular mechanism of the strong drought resistance of J24.
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Key words
RNA-Seq,Drought stress,MAPKKK21,Maize,Jing24
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