溃结核心方对溃疡性结肠炎小鼠NF-κB/HIF-1 α通路的影响
Journal of Emergency in Traditional Chinese Medicine（2023）
上海中医药大学附属曙光医院
Abstract
目的 探究溃结核心方对葡聚糖硫酸钠诱导的溃疡性结肠炎(UC)小鼠的保护作用和生效机制.方法 将48只C57BL/6J小鼠随机分为正常组、模型组、美沙拉嗪组及溃结核心方低、中、高剂量组.各组小鼠除正常组外均自由饮用4％DSS溶液共6 d以构建急性UC小鼠模型.记录小鼠一般情况、疾病活动指数评分,HE染色法进行病理检测并评分,记录各组小鼠结肠长度、病理评分结果;采用qRT-PCR和IHC法检测各组小鼠结肠组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)表达;分别使用qRT-PCR实验和WB实验检测缺氧诱导因子-1α(HIF-1α)、核转录因子-κB(NF-κB)的mRNA和蛋白表达水平.结果 相比正常组,模型组小鼠的体质量、结肠长度均明显降低(P＜0.01);DAI评分、病理组织学评分、TNF-α、IL-1β、NF-KB、HIF-1α mRNA和蛋白表达水平有显著升高(P＜0.05或P＜0.01);小鼠的结肠黏膜结构遭破坏,有炎性细胞浸润;与模型组相比,溃结核心方中、高剂量组和美沙拉嗪组小鼠体质量、结肠长度有明显增加(P＜0.05),DAI评分、病理组织学评分、TNF-α、IL-1 βmRNA表达水平均明显降低(P＜0.05或P＜0.01),溃结核心方高剂量组和美沙拉嗪组小鼠NF-κB、HIF-1αmRNA和蛋白表达水平均明显降低(P＜0.05或P＜0.01),结肠黏膜病理损伤明显减轻.结论 溃结核心方可显著改善DSS诱导的UC 小鼠的结肠炎症,其作用机制与调节NF-κB/HIF-1 α信号通路相关.
MoreKey words
Ulcerative colitis,Kuijie Hexin Decoction,Dextran sulfate sodium,NF-KB,HIF-1α,Mice
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