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利用人工miRNA沉默StIAA22对马铃薯根系构型的影响

Acta Horticulturae Sinica(2023)

甘肃农业大学省部共建干旱生境作物学国家重点实验室

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Abstract
通过检测26个StIAA家族基因在马铃薯根系发育过程中的表达发现:18个StIAA基因在根系成熟后的表达量比发育初期显著上调,8个StIAA基因变化不显著.利用人工microRNA(artificial microRNA,amiRNA)技术对上调表达最为显著的StIAA22做进一步的功能研究:构建干扰表达载体pB1121-amiRIAA,通过根癌农杆菌介导法转入马铃薯栽培品种'Dèsirèe'.经定量PCR检测,StIAA22在所有转基因株系中的表达均受到明显抑制;其根系形态构型与非转基因植株差异明显,根系生长受到抑制,根长明显变短,侧根数量增加,根系生物量减少.以上结果表明StIAA22在调节马铃薯根系形态建成中起关键作用.
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Key words
potato,StIAA22,artificial miRNA,gene silencing,rootarchitecture
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