Sequencing-based Functional Assays for Classification of BRCA2 Variants in Mouse ESCs.

Kajal Biswas,Alexander Y. Mitrophanov,Sounak Sahu,Teresa Sullivan,Eileen Southon,Darryl Nousome,Susan Reid, Sakshi Narula, Julia Smolen, Trisha Sengupta, Maximilian Riedel-Topper, Medha Kapoor, Anav Babbar,Stacey Stauffer,Linda Cleveland,Mayank Tandon,Tyler Malys,Shyam K. Sharan

CELL REPORTS METHODS（2023）

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Key words

BRCA2,breast cancer,variants of uncertain significance,VUS,bacterial artificial chromosome,BAC,recombineering,mouse ES Cells,DNA repair,cell viability,functional assay

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