大鼠颌下静脉丛采血技术方法及其效果比较
Chinese Journal of Comparative Medicine(2023)
山东中医药大学
Abstract
目的 介绍一种操作简便、可多次、大量采血的大鼠采血方法以及采血点的定位.方法 通过大鼠自身的体表特征,判定最佳进针点,使用注射器针头刺入动物颌下静脉丛缓慢抽取血液.结果 非麻醉状态下,单人平均77.46 s可完成一只大鼠的采血操作,平均采血量为0.53 mL;双人平均56.28 s可完成采血操作,平均采血量为0.59 mL.麻醉状态下,单人大鼠的采血操作平均耗时28.67 s,平均采血量为0.56 mL.结论 颌下静脉丛采血成功率高,对动物创伤小,且操作简单,采血量大,可作为药理毒理实验中大批量动物活体多次采血的优先选择.
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