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    • 抑制伪狂犬病病毒复制的宿主蛋白的筛选与鉴定

    HE Wenfeng,LI Chen,CHANG Hongtao, LI Longxi,CHEN Jing,YANG Guoqing,LIU Huimin

    Acta Veterinaria Et Zootechnica Sinica（2023）

    Cited 0|Views16
    Abstract
    本试验旨在利用TMT蛋白组学分析并筛选出抑制伪狂犬病病毒(pseudorabies virus,PRV)复制的关键宿主蛋白.前期通过TMT定量蛋白组学技术筛选出241个差异蛋白,Western blot和RT-qPCR验证4个差异表达的蛋白,结果与蛋白组学结果一致,表明蛋白组学结果真实可信.通过构建4个差异表达蛋白的真核表达载体,Western blot、RT-qPCR、病毒噬斑结果显示这4个宿主蛋白均可抑制PRV的复制;然后通过双荧光素报告基因检测IFN-β启动子活性,结果显示,MCCC1、STRAP促进IFN-β启动子活性效果更为显著;针对MCCC1和STRAP设计siRNA,Western blot和病毒噬斑结果显示,敲低STRAP显著促进PRV的复制.本试验最终筛选出显著影响PRV复制的宿主蛋白STRAP,为研究PRV与宿主互作的分子机制奠定基础.
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    Key words
    TMT proteomics,PRV,DEPs,STRAP
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    • Pretraining has recently greatly promoted the development of natural language processing (NLP)
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