茉莉花萜类合成酶基因的转录组鉴定及响应外源激素的表达研究
Biotechnology Bulletin（2022）
Abstract
探究萜类合成酶(Terpenoid synthase,TPS)在茉莉花萜类化合物合成中的作用及外源激素处理下的表达模式,为深入研究茉莉花TPS基因家族的功能及在外源激素胁迫中的作用奠定理论依据.以茉莉花转录组数据为基础,利用生物信息学方法对茉莉花TPS家族进行鉴定及分析,采用实时荧光定量分析茉莉花TPS家族在茉莉花不同组织中及不同激素处理下的表达模式.结果显示,茉莉花TPS家族含有8个同时具有Terpene_synthase和Terpene_synthase_C结构域的家族成员,分别命名为JsTPS1-JsTPS8.茉莉花TPS成员的蛋白长度为163-844个氨基酸;分子量为19 103.6-96 887.4 kD,主要定位于细胞质和叶绿体中.系统进化树显示茉莉花TPS基因家族可以分为4个亚家族(a、b、e/f、g);qRT-PCR结果显示,JsTPS1、JsTPS2、JsTPS3、JsTPS5、JsiTPS7基因在花瓣中高表达;经 IAA、GA、SA 和 MeJA 处理后 JsTPS1、JsTPS2、JsTPS3、JsTPS5、JsTPS6和 JsTPS7的表达量受到不同程度的诱导表达.MeJA处理可显著诱导6个茉莉花TPS基因的上调表达,相对表达量最大值出现在4-9 h;4种激素的诱导作用由大到小分别为:MeJA＞IAA＞GA＞SA.茉莉花含有8个TPS家族成员,茉莉花TPS基因主要在花瓣中高表达且与茉莉花香气形成关系密切,能够被IAA和MeJA诱导上调表达,GA和SA可能会抑制部分茉莉花TPS基因的表达.
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