PAPSS1的表达与脑胶质瘤临床指标和预后的相关性及对细胞增殖和凋亡的影响
Chinese Medicinal Biotechnology（2021）
Abstract
目的 研究PAPSS1表达与脑胶质瘤临床指标和预后的相关性,及其对细胞增殖和凋亡的影响.方法 采用免疫组化方法检测PAPSS1、PDL1蛋白在脑胶质瘤样本的表达,并纳入SPSS统计学分析.其中,PAPSS1和PDL1之间及其与临床指标的相关性分析采用Spearman法;生存期单因素分析采用Kaplan-Meier法和log-rank检验,将单因素分析中具有统计学意义的变量纳入COX多元回归生存分析.使用siRNA技术降低PAPSS1在脑胶质瘤细胞的表达.转染48 h后,利用MTT法检测细胞增殖比率,利用Hochest/PI法检测细胞凋亡率;PAPSS1、PDL1、ki67和Bcl2等基因的表达变化使用qPCR检测.上述实验数据采用GraphPad软件分析.P<0.05为有统计学意义.结果 IHC数据分析显示,癌组织PAPSS1浆表达和患者年龄、病理分级、复发等显著正相关,而PAPSS1核表达和各临床指标均无关,PDL1表达仅和年龄有关;PAPSS1浆表达和PDL1表达之间也显著正相关.生存分析显示,PAPSS1浆高表达的脑胶质瘤患者拥有更差的总生存期和无病生存期;PAPSS1核表达和PDL1表达都与预后无关.当使用siRNA技术降低了PAPSS1在癌细胞的表达时,细胞增殖比率出现下降而凋亡率上升,PDL1、ki67和Bcl2等基因的mRNA表达水平出现显著下调.结论 本研究发现PAPSS1浆表达与脑胶质瘤患者的年龄、病理分级、复发及更短的预后显著相关;降低PAPSS1表达能抑制细胞增殖并促进凋亡,同时也下调了PDL1、ki67、Bcl2等基因的表达水平,提示PAPSS1与脑胶质瘤的个性化治疗相关,值得进一步研究和探讨.
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