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N-糖链切除对重组抗狂犬病毒单克隆抗体中和活性的影响

CURRENT BIOTECHNOLOGY(2016)

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Abstract
重组抗狂犬病毒单克隆抗体是一种全人源的抗病毒感染单抗,主要用于狂犬病毒暴露后预防.采用糖苷酶处理2种重组抗狂犬病毒单克隆抗体Mab1、Mab2水解切除N-糖链,通过糖染色和毛细管电泳检测确定N-糖链酶解程度,利用快速荧光灶抑制试验检测其中和活性,分析N-糖链切除对该单抗中和活性的影响.结果显示,N-糖链切除后抗体中和活性无明显变化.说明N-糖链对这两株抗狂犬病毒单抗的体外中和活性不是必须的结构成分.
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