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Gas7在大鼠斜角带核发育过程中的表达

Chinese Journal of Histochemistry and Cytochemistry(2016)

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Abstract
目的 研究大鼠发育过程中,生长休止蛋白7(growth arrest-specific protein 7,Gas7)在斜角带核(diagonal band nucleus,DB)的表达.方法 应用RT-PCR方法、Western blot方法和免疫组织化学方法观察胚胎期16.5天(E16.5)、E20.5、出生当天(P0)、生后7天(P7)、P14、P21和2月龄(成年)大鼠DB的Gas7表达及定位.结果 RT-PCR和Western blot检测显示,在E16.5时Gas7表达最弱,其后逐渐增强,至P21时达到高峰;免疫组织化学染色显示,斜角带核水平支(horizontal limb of the diagonal band,HDB)在E16.5和E20.5时出现Gas7免疫反应阳性产物,Gas7阳性神经元出现于P0,至P21时阳性神经元数量最多,染色最深;斜角带核垂直支(vertical limb of the diagonal band,VDB)至P14时Gas7阳性神经元最多,P21和成年均有所减少.结论 Gas7的表达在大鼠DB发育过程中具有时间和空间上的特异性,提示Gas7可能参与大鼠DB的发育过程.
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Key words
Growth arrest-specific protein 7,development,diagonal band nucleus,rat
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