附子茎段组培快繁体系的建立
Molecular Plant Breeding（2020）
Abstract
为探讨附子丛生芽的诱导、增殖及不定根诱导条件.本研究以附子带腋芽茎段为外植体,以MS和1/2MS为基本培养基,添加不同浓度的6-BA、NAA、TDZ、IBA等植物生长调节剂,观察附子丛生芽的诱导、增殖、生根情况.结果 发现,附子茎段经75％乙醇处理30s后,0.1％ HgC12灭菌10 min,污染率为27.78％,存活率可达84.61％.附子第三个腋芽,诱导率为53.34％,死亡外植体少.丛生芽在MS+6-BA 2 mg/L+NAA0.3 mg/L条件时诱导率为86.67％,芽长1.947cm,植株茎干粗壮.增殖培养时添加TDZ 2 mg/L+NAA0.3 mg/L,增殖系数达到4.029,苗粗壮,叶片浓绿.生根培养基条件为1/MS+IBA 0.5 mg/L时,15d的生根率可达100％,平均根长0.906 cm,平均根数10.5条,叶色翠绿,生长旺盛.研究表明,最佳的取材部位为第三个腋芽,丛生芽诱导的最佳培养基为MS+6-BA 2 mg/L+NAA 0.3 mg/L-,丛生芽增殖培养基中添加TDZ 2 mg/L+NAA 0.3 mg/L的增殖效果最好,适宜的生根培养基为1/2MS+IBA 0.5 mg/L.本研究为附子快繁体系的建立和工厂化育苗提供了理论依据.
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