Expertise Summary
Molecular Pharmacology of GPCRs
Fluorescence Correlation Spectroscopy (FCS, FCCS, RICS, PCH)
Single cell pharmacology (binding and function)
Live cell confocal microscopy (fluorescent ligand binding, receptor trafficking)
Advanced imaging approaches (FLIM, FRAP)
Single cell calcium measurements
Cell microinjection
Radioligand binding
Second messenger assays (inositol phosphates, cAMP)
Western blotting and immunoprecipitation
I am particularly focussed on G protein-coupled receptors, and the application of advanced microscopy to study their pharmacology at the single and sub-cellular level.